منابع مشابه
Hela Cell Plasma Membranes
A method for the preparation of HeLa cell plasma membrane ghosts is described. The purity of the plasma membrane fraction was examined by phase contrast and electron microscopy, by chemical analysis, and by assay of marker enzymes. Data on the composition of the plasma membrane fraction are given. It was observed that the distribution pattern of 5'-nucleotidase activity among the subcellular fr...
متن کاملEditorial: HeLa Cell Sequela
Free and informed consent of participants in research studies is a cornerstone of biomedical research, but this has not always been the case. Cells derived from tumor tissue of an American woman without her consent more than 60 years ago and named HeLa cells were eventually identified as belonging to Henrietta Lacks, a fact that became widely known in 2010 upon publication of a bestselling book...
متن کاملSubcompartments in Intact HeLa Cells
Two recombinant aequorin isoforms with different Ca 2 1 affinities, specifically targeted to the endoplasmic reticulum (ER), were used in parallel to investigate free Ca 2 1 homeostasis in the lumen of this organelle. Here we show that, although identically and homogeneously distributed in the ER system, as revealed by both immunocytochemical and functional evidence, the two aequorins measured ...
متن کاملRna Synthesis in Hela Cells
Interphase HeLa cells manifest a stepwise shutoff of RNA synthesis when the tonicity of the extracellular medium is gradually increased. Synthesis of heterogeneous nuclear RNA is most sensitive and is selectively inhibited at 1.5 times isotonicity (450 milliosmols/liter), while 45S ribosomal RNA synthesis is not affected significantly below 2.0 times isotonicity. Transfer RNA synthesis is least...
متن کاملTurnover of Hela Ribosomal Rna
A class of RNA in HeLa cytoplasm with a sedimentation value of 22S is described. This RNA is a true cytoplasmic component and is not an artifact of cell rupture or of the method of RNA preparation. The 22S RNA sediments in a sucrose gradient with those ribosomal structures containing 28S ribosomal RNA (60S and 74S particles and polyribosomes). It has a base composition and methyl content simila...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: ICES Journal of Marine Science
سال: 1978
ISSN: 1054-3139,1095-9289
DOI: 10.1093/icesjms/38.1.1